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On-site TMT labeled <t>frozen</t> <t>prostate</t> <t>cancer</t> <t>tissue</t> slide. A Bright-field scanning of the adjacent prostate cancer H&E slide annotated with normal (yellow), Gleason 3 (cyan), Gleason 4 (blue), and Gleason 5 (purple) regions. B Principal component analysis of Gleason score regions based on the protein expression profiles. C Hierarchical clustering based on the expression profiles of 289 proteins across different Gleason score regions. D Significantly changed proteins (absolute log2 fold change > 1, p-value < 0.05) from pairwise comparison of two different Gleason score regions
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On-site TMT labeled <t>frozen</t> <t>prostate</t> <t>cancer</t> <t>tissue</t> slide. A Bright-field scanning of the adjacent prostate cancer H&E slide annotated with normal (yellow), Gleason 3 (cyan), Gleason 4 (blue), and Gleason 5 (purple) regions. B Principal component analysis of Gleason score regions based on the protein expression profiles. C Hierarchical clustering based on the expression profiles of 289 proteins across different Gleason score regions. D Significantly changed proteins (absolute log2 fold change > 1, p-value < 0.05) from pairwise comparison of two different Gleason score regions
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On-site TMT labeled <t>frozen</t> <t>prostate</t> <t>cancer</t> <t>tissue</t> slide. A Bright-field scanning of the adjacent prostate cancer H&E slide annotated with normal (yellow), Gleason 3 (cyan), Gleason 4 (blue), and Gleason 5 (purple) regions. B Principal component analysis of Gleason score regions based on the protein expression profiles. C Hierarchical clustering based on the expression profiles of 289 proteins across different Gleason score regions. D Significantly changed proteins (absolute log2 fold change > 1, p-value < 0.05) from pairwise comparison of two different Gleason score regions
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On-site TMT labeled <t>frozen</t> <t>prostate</t> <t>cancer</t> <t>tissue</t> slide. A Bright-field scanning of the adjacent prostate cancer H&E slide annotated with normal (yellow), Gleason 3 (cyan), Gleason 4 (blue), and Gleason 5 (purple) regions. B Principal component analysis of Gleason score regions based on the protein expression profiles. C Hierarchical clustering based on the expression profiles of 289 proteins across different Gleason score regions. D Significantly changed proteins (absolute log2 fold change > 1, p-value < 0.05) from pairwise comparison of two different Gleason score regions
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On-site TMT labeled <t>frozen</t> <t>prostate</t> <t>cancer</t> <t>tissue</t> slide. A Bright-field scanning of the adjacent prostate cancer H&E slide annotated with normal (yellow), Gleason 3 (cyan), Gleason 4 (blue), and Gleason 5 (purple) regions. B Principal component analysis of Gleason score regions based on the protein expression profiles. C Hierarchical clustering based on the expression profiles of 289 proteins across different Gleason score regions. D Significantly changed proteins (absolute log2 fold change > 1, p-value < 0.05) from pairwise comparison of two different Gleason score regions
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On-site TMT labeled <t>frozen</t> <t>prostate</t> <t>cancer</t> <t>tissue</t> slide. A Bright-field scanning of the adjacent prostate cancer H&E slide annotated with normal (yellow), Gleason 3 (cyan), Gleason 4 (blue), and Gleason 5 (purple) regions. B Principal component analysis of Gleason score regions based on the protein expression profiles. C Hierarchical clustering based on the expression profiles of 289 proteins across different Gleason score regions. D Significantly changed proteins (absolute log2 fold change > 1, p-value < 0.05) from pairwise comparison of two different Gleason score regions
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On-site TMT labeled <t>frozen</t> <t>prostate</t> <t>cancer</t> <t>tissue</t> slide. A Bright-field scanning of the adjacent prostate cancer H&E slide annotated with normal (yellow), Gleason 3 (cyan), Gleason 4 (blue), and Gleason 5 (purple) regions. B Principal component analysis of Gleason score regions based on the protein expression profiles. C Hierarchical clustering based on the expression profiles of 289 proteins across different Gleason score regions. D Significantly changed proteins (absolute log2 fold change > 1, p-value < 0.05) from pairwise comparison of two different Gleason score regions
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On-site TMT labeled <t>frozen</t> <t>prostate</t> <t>cancer</t> <t>tissue</t> slide. A Bright-field scanning of the adjacent prostate cancer H&E slide annotated with normal (yellow), Gleason 3 (cyan), Gleason 4 (blue), and Gleason 5 (purple) regions. B Principal component analysis of Gleason score regions based on the protein expression profiles. C Hierarchical clustering based on the expression profiles of 289 proteins across different Gleason score regions. D Significantly changed proteins (absolute log2 fold change > 1, p-value < 0.05) from pairwise comparison of two different Gleason score regions
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On-site TMT labeled frozen prostate cancer tissue slide. A Bright-field scanning of the adjacent prostate cancer H&E slide annotated with normal (yellow), Gleason 3 (cyan), Gleason 4 (blue), and Gleason 5 (purple) regions. B Principal component analysis of Gleason score regions based on the protein expression profiles. C Hierarchical clustering based on the expression profiles of 289 proteins across different Gleason score regions. D Significantly changed proteins (absolute log2 fold change > 1, p-value < 0.05) from pairwise comparison of two different Gleason score regions

Journal: Clinical Proteomics

Article Title: SPOT: spatial proteomics through on-site tissue-protein-labeling

doi: 10.1186/s12014-024-09505-5

Figure Lengend Snippet: On-site TMT labeled frozen prostate cancer tissue slide. A Bright-field scanning of the adjacent prostate cancer H&E slide annotated with normal (yellow), Gleason 3 (cyan), Gleason 4 (blue), and Gleason 5 (purple) regions. B Principal component analysis of Gleason score regions based on the protein expression profiles. C Hierarchical clustering based on the expression profiles of 289 proteins across different Gleason score regions. D Significantly changed proteins (absolute log2 fold change > 1, p-value < 0.05) from pairwise comparison of two different Gleason score regions

Article Snippet: Fresh frozen prostate cancer tissue samples were obtained from JHU Pathology Core/Biospecimen Bank with approval from the Institutional Review Board of Johns Hopkins Medical Institutions.

Techniques: Labeling, Expressing, Comparison

Journal: Cell Stem Cell

Article Title: Development, maturation, and maintenance of human prostate inferred from somatic mutations

doi: 10.1016/j.stem.2021.02.005

Figure Lengend Snippet:

Article Snippet: Snap-frozen human prostate post-mortem biopsies , Amsbio Biorepository , https://www.amsbio.com/products/biorepository.

Techniques: Recombinant, DNA Extraction, Sequencing, Modification, Software, Variant Assay